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Author: Admin | 2025-04-28
Using OX-42/CD11b/c) in male rats at 16 months old with no treatment, with losartan, or with hydrochlorothiazide; scale bar (10X) = 200 μm; scale bar (40X) = 50 μm. Representative image for 16 month old male is also used in Fig. 2E. D Number of active microglia per 80,000 μm2 in level 2 of the PVN in male rats at 16-months-old with no treatment, with losartan, or with hydrochlorothiazide (n = 6/group). Data used for 16 month old male is also used in Fig. 2F. E Total number of microglia per 80,000 μm.2 in level 2 of the PVN in male rats at 16-months-old with no treatment, with losartan, or with hydrochlorothiazide (n = 6/group); Data used for 16 month old male is also used in Fig. 2G. F Sholl analysis expressed as number of intersections as a function of distance from the soma (μm) in 8 microglia per rat from level 2 of the PVN in 16- vs. 16 month old + losartan male (M) rats (n = 6/group); Data used for 16 month old male is also used in Fig. 2L, M. G Sholl analysis expressed as number of intersections as a function of distance from the soma (μm) in 8 microglia per rat from level 2 of the PVN in 16- vs. 16 month old + hydrochlorothiazide male (M) rats (n = 6/group); Data used for 16 month old male is also used in Fig. 2L, M. H Representative photomicrographs (captured at 10X magnification with 20X magnification inset) from level 2 of the PVN (bregma − 1.72 to − 2.04 mm) of astrocytes (stained using glial fibrillary acidic protein (GFAP)) in male rats at 16 months old with no treatment, with losartan, or with hydrochlorothiazide; scale bar (10X) = 200 μm; scale bar (20X) = 50 μm. Representative image for 16 month old male is also used in Fig. 2N. I GFAP expression presented as percent area of level 2 of the PVN in male rats at 16-months-old with no treatment, with losartan, or with hydrochlorothiazide (n = 6/group); data used for 16 month old male is also used in Fig. 2O. J Representative photomicrographs of interleukin-6 (IL-6) expression in level 2 of the PVN (bregma − 1.72 to − 2.04 mm) of male rats at 16 months old with no treatment or with losartan (N = 5–6/group). Scale bar (10X) = 200 μm; representative image for
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